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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
19/12/2023 |
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Data da última atualização: |
29/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, K. S.; COSTA, C.; BESSA, M. J.; TEIXEIRA, J. P.; SILVA, A. V. C. da; PADILHA, F. F.; DARIVA, C.; OLIVEIRA, M. B. P. P. |
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Afiliação: |
KLEBSON SILVA SANTOS, ITP; CARLA COSTA, Portuguese National Institute of Health; MARIA JOÃO BESSA, Universidade do Porto; JOAO PAULO TEIXEIRA, UNIVERSIDADE DO PORTO; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FRANCINE FERREIRA PADILHA, ITP; CLÁUDIO DARIVA, ITP; MARIA BEATRIZ PINTO PRIOR OLIVEIRA, UNIVERSIDADE DO PORTO. |
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Título: |
Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Exploration of Foods and Foodomics, v. 1, p. 130-142, 2023. |
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DOI: |
https://doi.org/10.37349/eff.2023.00011 |
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Idioma: |
Inglês |
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Conteúdo: |
Aim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. MenosAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negati... Mostrar Tudo |
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Thesagro: |
Conservação; Nim; Planta Medicinal. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02468naa a2200253 a 4500
001 2160010
005 2023-12-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.37349/eff.2023.00011$2DOI
100 1 $aSANTOS, K. S.
245 $aAzadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage.$h[electronic resource]
260 $c2023
520 $aAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells.
650 $aConservação
650 $aNim
650 $aPlanta Medicinal
700 1 $aCOSTA, C.
700 1 $aBESSA, M. J.
700 1 $aTEIXEIRA, J. P.
700 1 $aSILVA, A. V. C. da
700 1 $aPADILHA, F. F.
700 1 $aDARIVA, C.
700 1 $aOLIVEIRA, M. B. P. P.
773 $tExploration of Foods and Foodomics$gv. 1, p. 130-142, 2023.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/01/2005 |
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Data da última atualização: |
11/07/2005 |
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Autoria: |
COUTINHO, M. V.; COSTA, M. F. da; CABRAL, G. B.; SÁ, M. F. G. de. |
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Título: |
Regeneração de plantas de feijão azuki (Vigna Angularis) via organogênese direta. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2003. |
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Páginas: |
10 p. |
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Série: |
(Embrapa Recursos Genéticos e Biotecnologia. Circular Técnica, 27). |
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Idioma: |
Português |
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Thesagro: |
Biologia Molecular; Feijão Azuki; Melhoramento Genético Vegetal; Praga de Planta; Regeneração; Vigna Angularis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CENARGEN/24693/1/ct027.pdf
https://www.cenargen.embrapa.br/publica/trabalhos/ct027.pdf
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Marc: |
LEADER 00742nam a2200229 a 4500
001 1185433
005 2005-07-11
008 2003 bl uuuu u0uu1 u #d
100 1 $aCOUTINHO, M. V.
245 $aRegeneração de plantas de feijão azuki (Vigna Angularis) via organogênese direta.
260 $aBrasília: Embrapa Recursos Genéticos e Biotecnologia$c2003
300 $a10 p.
490 $a(Embrapa Recursos Genéticos e Biotecnologia. Circular Técnica, 27).
650 $aBiologia Molecular
650 $aFeijão Azuki
650 $aMelhoramento Genético Vegetal
650 $aPraga de Planta
650 $aRegeneração
650 $aVigna Angularis
700 1 $aCOSTA, M. F. da
700 1 $aCABRAL, G. B.
700 1 $aSÁ, M. F. G. de
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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